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AXL expression of breast cancer cell lines and cell binding assay. (a) Total AXL protein levels in breast cancer cells were determined by western blotting. The two TNBC cell lines, MDA-MB-231 and Hs578T, had high levels of AXL protein expression. (b) Flow cytometry showed high levels of AXL expression on the surface of MDA-MB-231 and Hs578T human TNBC cells. (c) Competitive cell binding assay showed that 64Cu-anti-hAXL bound to MDA-MB-231 cells with an IC50 value of 0.76 nM.
