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. 2017 Apr 23;2017:4035160. doi: 10.1155/2017/4035160

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Copyright © 2017 Sarah Sutter et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Enzymatic, immunohistochemical, and immunofluorescent staining on sections of femurs and tibias after contrast enhancement. (a) TRAP staining showing multinucleated osteoclasts (red) in the epiphyseal gap. (b) CD31 staining showing a small ink-gelatin contrasted vessel (star) with positively stained endothelial lining (arrows). (c) Immunohistochemistry for osteoblasts (arrow) in the corticalis. (d) Immunofluorescence for CD68 showing single rat macrophages (arrows) inside the bone marrow (plus).