Table 2. Effect of metal ions, solvents and detergents on PleoLip241 and PleoLip369 activities.
Standard deviations were less than 5%.
| Residual activity (100%) | ||||
|---|---|---|---|---|
| PleoLip241 | PleoLip369 | |||
| Metal ions | 1 mM | 10 mM | 1 mM | 10 mM |
| NaCl | 102 | 96 | 100 | 20 |
| MgCl2 | 92 | 91 | 88 | 15 |
| CaCl2 | 101 | 84 | 98 | 6 |
| ZnCl2 | 83 | 37 | 86 | 0 |
| KCl | 88 | 83 | 55 | 7 |
| CuSO4 | 50 | 0 | 35 | 0 |
| FeSO4 | 50 | 20 | 27 | 0 |
| MnCl2 | 100 | 95 | 77 | 18 |
| Solvents | 10% | 20% | 10% | 20% |
| Ethanol | 70 | 70 | 34 | — |
| Glycerol | 81 | 68 | 55 | 52 |
| Aceton | 45 | 40 | 20 | — |
| Methanol | 70 | 75 | 40 | — |
| Methylacetate | 65 | 50 | — | — |
| t-Buthanol | 80 | 65 | 62 | 35 |
| Ethyl acetate | 63 | 72 | 45 | 22 |
| Detergents | 1% | 1% | ||
| Tween20 | 0 | 0 | ||
| Tween 80 | 18 | 0 | ||
| Triton X100 | 135 | 115 | ||
| SDS | 0 | 0 | ||