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High Pi loading enhanced inflammatory response in LPS-stimulated RAW264.7 cells. RAW264.7 cells were preincubated with Pi (2 mM) for 12 h, followed by an incubation with LPS (1 µg/µl) for 1 h. Total RNA was extracted and relative TNF-α (A) and MCP-1 (B) mRNA levels was determined by quantitative real-time RT-PCR. Data are expressed as mean ± SEM (n = 3). *p<0.05 vs Pi: 0.9 mM; #p<0.05 vs Pi: 0.9 mM + LPS.
