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High Pi loading enhanced ROS production in RAW264.7 cells. (A) After incubation with APF (20 µM) for 30 min, cells were treated by different concentrations of Pi for 2 h with or without various oxidase inhibitors. (B, C) RAW264.7 cells were pretreated with DPI (10 µM) for 30 min, and then treated with Pi (2 mM) for 1 h. Data are expressed as mean ± SEM (n = 5). *p<0.05 vs Pi: 0.9 mM; #p<0.05 vs Pi: 2 mM.
