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Total ion chromatogram of LC-MS/MS of recombinant L-asparaginase. Protein was resolved on a 12% SDS PAGE excised and digested with trypsin. Protein was dissolved in 0.1% Formic acid and the chromatogram was obtained from the LC-MS instrument. The separation of digested protein as peaks in respective time (min), mass to charge ratio obtained for the peaks analysed using the MS instrument
