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Purification steps for recombinant l-asparaginase from Lactobacillus reuteri DSM 20016 overexpressed in E. coli
| Step | Total protein (mg/ml) | Total activity (U) | Specific activity (U/mg) | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
| Crude | 1360 | 78.24 | 0.06 | 100 | 1 |
| Ni–NTA | 114.4 | 72.08 | 0.63 | 92 | 10.5 |
