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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Exp Neurol. 2017 Aug 7;297:101–109. doi: 10.1016/j.expneurol.2017.08.002

Figure 3. Mutations in HSPB1 attenuate non-cell autonomous motor neuron protection.

Figure 3

A. MN survival at day 6 of MN co-culture assay with wild type, SOD1(G93A), SOD1(G93A) + HSPB1(WT), SOD1(G93A) + HSPB1(G84R), SOD1(G93A) + HSPB1(R136W) astrocytes. MN survival was normalized to counts from MNs cultured with wild type astrocytes. (n = 3 for all groups, each n was run in triplicate.) B. Representative immunoblot confirming the expression of human HSPB1 constructs in SOD1(G93A) astrocytes. Western blots were probed with an antibody specific to human HSPB1 C. Representative images of HB9-GFP expressing MNs (shown in black) after 6 days in co-culture with wild type astrocytes, SOD1(G93A) astrocytes, and SOD1(G93A) astrocytes transduced with HSPB1(WT), HSPB1(G84R) or HSPB1(R136W). D. MN survival at day 6 of MN co-culture assay with wild type, WT+RFP, WT + HSPB1 (WT), WT + HSPB1(G84R), WT + HSPB1(R136W) astrocytes. MN survival was normalized to counts from MNs cultured with wild type astrocytes. (n = 3 for all groups, each n was run in triplicate.) E. Representative immunoblot confirming the expression of HSPB1 constructs in wild type astrocytes using an antibody specific to human HSPB1. Error bars denote s.e.m. ***P<0.0005, *P<0.01, n.s., non-significant.