Fig. 1.
Mapping of B cell and T cell epitopes of hα-Syn in C57BL/6 mice immunized with plasmid encoding full length hα-Syn protein followed by electroporation (A, C) or full length hα-Syn recombinant protein formulated with AdvaxCpG adjuvant (B, D). (A, B) B cell epitopes were mapped by analyzing the binding of immune sera (1:1000 for DNA immunized sera and 1:5000 for protein immunized sera) to overlapping hα-Syn peptides or irrelevant peptide by ELISA. (C, D) Th cell responses were detected in splenocytes of immunized mice in vitro re-stimulated with the same overlapping peptides.