Fig. 3.
Schematic representation of DNA hα-Syn vaccine constructs and expression in transfected CHO cells. (A) Strategy for cloning genes encoding several hα-Syn B cell epitope/s fused with MultiTEP into the pVAX1 vector and schematic representation of PV-1947D, PV-1948D, PV-1949D, and PV-1950D constructs. (B–D) The expression of PV-1947D (B), PV-1948D (C), PV-1949D (D), and PV-1950D (B–D) and secretion of protein was demonstrated by WB of conditioned media of transiently transfected CHO cells. Proteins were visualized by staining with anti-hα-Syn85–99 Abs (A), anti-α-hSyn109–126 Abs (B), and anti-α-hSyn126–140 Abs (C) followed by HRP conjugated anti-mouse IgG.