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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Neurobiol Aging. 2017 Aug 10;59:156–170. doi: 10.1016/j.neurobiolaging.2017.08.006

Fig. 6.

Fig. 6

DNA vaccines induced IgG1, IgG2ab and IgG2b antibodies specific to recombinant hα-Syn and hα-Syn synthesized by SH-SY5Y/hα-Syn cells. (A–D) Endpoint titers of antibodies specific to recombinant hα-Syn protein were detected in sera collected after the third immunization with PV-1947D (p3hα-Syn85–99-MultiTEP; A), PV-1948D (p3hα-Syn109–126-MultiTEP; B), PV-1949D (p3hα-Syn126–140-MultiTEP; C), PV-1950D (p3hα-Syn126–140-3hα-Syn109–126-3hα-Syn85–99-MultiTEP; D). (E–G) Staining of SH-SY5Y/α-Syn and SH-SY5Y/WT control cell lysates with purified anti-hα-Syn antibodies: anti-hα-Syn85–99 (E), anti-hα-Syn109–126 (F), and anti-hα-Syn126–140 (G). Full recombinant hα-Synuclein protein was loaded as a positive control. (H–K) Isotypes of antibodies generated by DNA vaccines. Sera from individual mice were diluted 1:1000.

HHS Vulnerability Disclosure