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. 2017 Sep 25;7:12272. doi: 10.1038/s41598-017-11129-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Connexin 43 expression, relative to GAPDH, in human fetal hearts aged 67–137 DGA. (A) Cx43 expression is significantly increased in the mid-second trimester (107–136 DGA) when compared to 67–73 DGA and 96 DGA. (B) Example Western blot of Cx43 expression across gestation, and the GAPDH loading control, which did not demonstrate developmental regulation. (**p = 0.001 and ***p = 0.0004).