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. 2017 Sep 25;7:12272. doi: 10.1038/s41598-017-11129-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The absence of Connexin 43 expression in one human fetal heart aged 97 DGA. In the process of testing Cx43 expression in the fetal heart samples, we discovered that one heart aged 97 DGA lacked any detectable Cx43 expression (left, top, sample 3). We confirmed this across three separate gels. Despite the lack of Cx43, the GAPDH loading control produced a clear band (left, bottom). At comparative ages (e.g. 73–107 DGA) Cx43 expression was clearly detectable in all other samples (right, top; blots from Fig. 4).