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. 2017 Sep 25;7:12262. doi: 10.1038/s41598-017-12135-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-culture of primary CLL cells with BMSC, CD40L and CpG ODN induces the expression of miR-21 and downregulation of the miR-21 targets PTEN, PDCD4 and PIAS3. (a) Primary CLL cells from 40 patients were cultured in suspension or in co-culture for 48 hours and miR-21 expression was measured by QRT-PCR. (b) Primary CLL cells from 22 patients were cultured in suspension or in co-culture for 48 hours. PTEN, PDCD4 and PIAS3 expression was determined by QRT-PCR. (c–d) Relative gene expression comparison between matched samples from peripheral blood (PB) and lymph nodes (LN) from 17 treatment-naive CLL patients, using public data from Y.Herishanu et al., Blood 2011²³. mRNA expression level of miR-21 (c) and PTEN (d) were analyzed and compared by Wilcoxon matched pairs signed rank test. (e) PBMC from 13 patients were pre-treated with 5 µM PCI-32765 for 1 hour and cultured in suspension or in co-culture for 48 hours. Expression levels of miR-21 were measured by QRT-PCR. *P < 0.05, **P < 0.01, ***P < 0.0001.