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. 2017 Sep 25;3:21. doi: 10.1038/s41522-017-0029-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Predicted relative gene family abundances for stream biofilms in different habitats. a Boxplots displaying the relative gene counts corresponding to KEGG orthology families from category 2 of biofilms from glacier-fed streams (blue), headwaters (red) and mid-reaches (green). Asterisks (red) indicate gene categories that are significantly different (ANOVA, Tukey HSD, Bonferroni-corrected p < 0.05) between biofilms from glacier-fed streams and subalpine streams. Boxes indicate the interquartile ranges, medians are shown as lines within the boxes, whiskers display lower and upper quartiles. b Boxplots displaying the relative gene counts corresponding to the KEGG orthology families Metabolism (shown on upper y-axis, blue), Environmental Information Processing and Cellular Processes (both shown on lower y-axis, red). Boxes indicate the interquartile ranges, medians are shown as lines within the boxes, whiskers display lower and upper quartiles, outliers are shown as circles. Asterisks (black) highlight significant differences in gene counts between biofilms from glacier-fed streams and subalpine streams without glacier influence (ANOVA, Tukey HSD, p < 0.01)