Figure 6.

Hepatic fibrosis associated with glucose intolerance, increased glucose synthesis and storage in high AGE fed DDOST+/− mice. (A) Glycated haemoglobin. (B) Fasting plasma insulin concentrations. (C) Plasma glucose curve over 120 mins following a 1IU/kg insulin bolus (ipITT) and area-under-the-curve (AUC) analysis. (D) Hepatic glycogen measured in liver tissue. (E) SWATH-MS protein intensities for the glycogen storage pathway protein (GLGB). (F) qPCR of Pck2, the enzyme involved in gluconeogenesis. (G) Heat map representation of SWATH-MS proteomics data for enzymatic pathways involved in GNG. Significant proteins are represented as the Log₂ fold change where red indicates a decreased and blue indicates an increase in protein concentrations. Data represented as means ± SD (n = 4–9/group). For proteomics, MSstatsV3.5.1 determined significant (P < 0.05) log fold changes in the protein intensities between the selected experimental group the WT low AGE diet group. *P < 0.05, student’s t-test. Genotype effect P < 0.05, (diet effect) P < 0.05, 2-way ANOVA and multiple comparison of genotype, diet and interaction by Bonferroni’s post hoc test.