Table 3.

Changes of the activity of enzymes in rumen fluid of goats.

Parameter		Groups				P-Value
		I	II	III	IV	I VS II	III VS IV	I VS III	II VS IV
CMCase (U/mL)	0d	74.39 ± 1.87	73.50 ± 1.50	73.13 ± 1.39	73.20 ± 1.38	0.54	0.79	0.453	0.824
	20d	85.89 ± 2.11	81.03 ± 2.51	89.38 ± 3.21	88.15 ± 1.45	0.15	0.626	0.099	0.016a
	60d	112.06 ± 3.33	109.07 ± 3.22	67.97 ± 2.10	71.97 ± 2.43	0.266	0.186	0.001a	0.002a
Xylanase (U/mL)	0d	10.03 ± 0.33	10.02 ± 0.41	10.24 ± 0.47	10.20 ± 0.35	0.975	0.701	0.424	0.216
	20d	14.56 ± 0.40	18.14 ± 1.27	15.74 ± 1.97	11.27 ± 1.15	0.016a	0.019a	0.246	0.013a
	60d	21.25 ± 0.64	34.57 ± 2.35	26.73 ± 4.34	19.94 ± 1.19	0.001a	0.084	0.073	0.005a
Pectinase (U /mL)	0d	45.51 ± 3.01	45.15 ± 2.14	45.14 ± 1.65	45.16 ± 1.71	0.728	0.684	0.829	0.992
	20d	37.42 ± 4.56	60.04 ± 1.87	44.23 ± 2.70	47.36 ± 1.34	0.002a	0.033a	0.013a	0.001a
	60d	17.19 ± 2.57	26.69 ± 0.53	20.13 ± 2.49	21.01 ± 2.25	0.016a	0.433	0.275	0.013a
β-glucosidase (U/ mL)	0d	72.62 ± 3.31	72.52 ± 3.23	71.90 ± 2.48	72.05 ± 2.40	0.954	0.75	0.68	0.407
	20d	68.90 ± 4.03	62.40 ± 2.67	60.79 ± 2.69	66.18 ± 3.98	0.168	0.177	0.005a	0.006a
	60d	59.89 ± 0.49	55.97 ± 2.79	50.82 ± 3.54	59.33 ± 3.61	0.13	0.051	0.016a	0.004a
Protease (µg /min.mL−1)	0d	3.25 ± 0.80	3.14 ± 0.35	3.15 ± 0.30	3.17 ± 0.28	0.8	0.571	0.827	0.58
	20d	3.28 ± 0.66	3.18 ± 0.26	2.74 ± 0.62	3.20 ± 0.35	0.775	0.095	0.244	0.957
	60d	4.49 ± 0.43	4.42 ± 0.16	4.16 ± 0.32	4.72 ± 0.48	0.839	0.164	0.471	0.411
Amylase (U/dL)	0d	20.92 ± 0.78	20.89 ± 0.37	20.76 ± 1.17	20.78 ± 0.68	0.962	0.967	0.854	0.778
	20d	24.88 ± 0.33	21.17 ± 1.50	25.59 ± 0.83	25.92 ± 0.61	0.029a	0.244	0.25	0.024a
	60d	27.62 ± 0.59	25.02 ± 0.58	26.26 ± 1.14	27.71 ± 1.04	0.006a	0.163	0.145	0.033a
Lipase (U/ L)	0d	19.24 ± 1.69	18.99 ± 1.09	19.92 ± 1.40	19.85 ± 1.38	0.862	0.861	0.316	0.529
	20d	18.81 ± 1.12	23.05 ± 1.36	18.25 ± 2.48	18.76 ± 0.69	0.037a	0.634	0.742	0.007a
	60d	21.13 ± 2.32	30.50 ± 3.37	32.42 ± 4.18	33.38 ± 3.82	0.041a	0.713	0.014a	0.468

^aMean significant difference (P < 0.05).