Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 25;7:12232. doi: 10.1038/s41598-017-12001-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Correlation of RSV isolate replication kinetics with patient sample titre. (a) Immortalized HEp-2 cells were inoculated with patient nasopharyngeal (NP) samples that were determined to be RSV type-A or type-B positive by the NxTAG RVP Luminex panel at PROVLab, Alberta, Canada. The HEp-2 cells were fixed and immunostained for RSV infection two days later. The morphology and size of the plaques were analyzed using automated color segmentation software on a digital phase contrast microscope. There was a range of sizes of foci of cell culture infection among the samples. (b) Comparison of RSV patient sample titres were determined by qRT-PCR and the number of focus forming units per mL. Infectivity index was determined by the dividing the infectious titre (FFU/mL) by the genome equivalents/mL (by qRT-PCR). (c) A replication time course over 5 days of RSV type-A and type-B isolates in immortalized cells. HEp-2 cells were inoculated by equal inputs of genomic equivalents as determined by qRT-PCR of two high titre, medium titre and low titre isolates of RSV type-A and RSV type-B to determine whether there was a difference in replication kinetics of isolates of different titres in patient samples. The number in parentheses beside the sample identifier indicates the RSV titre in the original patient’s sample. Inset below: viral titres 24 hrs post infection. (d), RSV type-A and B titres were determined by qRT-PCR and plotted versus the area of foci of infection after 48 hours on HEp-2 cells. A line of best fit is shown for RSV type-A and type-B.