Figure 5.

Comparison of RSV phylogenetics with age and patient NP sample titre during the 2014–2015 and 2015–2016 seasons in Alberta Canada. The phylogenetic trees were constructed by a neighbor-joining method using Geneious software from a Geneious alignment plug-in tool. (a) RSV type-A in Alberta strains sequenced from 2014–2016. (b) RSV type-B in Alberta strains sequenced from 2014–2016. Patient age indicated by red (<1 year), orange (1–18 years) or purple (>65 years). Patient nasopharyngeal sample viral loads indicated by ▲ (10⁶–10⁸ RNA copies/mL), ● (10⁴–10⁵ RNA copies/mL), or ■ (10²–10³ RNA copies/mL). The green box encompassing the isolates at the top of the tree indicates the resident clades which are lower titre isolates. The blue box encompassing the isolates on the bottom of the figure indicates the HiT clade as described in the results section. P values note the t-test comparison between the viral load of isolates of the resident clades versus the HiT clade.