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. 2017 Sep 25;7:12289. doi: 10.1038/s41598-017-12523-z

Figure 2.

Figure 2

Infection of silkworms with germinated conidia of A. vanbreuseghemii TIMM2789 dermatophytes. (a) Microscopic observation of conidia of A. vanbreuseghemii TIMM2789 dermatophytes (upper panel) and germinated conidia cultured at 28 °C for 26 h (lower panel). (b) Silkworms reared at 30 °C for 72 h after injection of 4 × 106 of germinated conidia (right). Saline control (left). n = 10/group. (c) Conidia of A. vanbreuseghemii TIMM2789 dermatophytes were cultured at 28 °C for 26 h (germinated conidia). Conidia (4 × 106) or germinated conidia (4 × 106) were injected into the silkworm hemolymph, and the silkworms were reared at 30 °C. Survival of the animals was monitored. n = 5/group. (d) Conidia of A. vanbreuseghemii TIMM2789 dermatophytes were cultured at 28 °C for 26 h. Dermatophytes (0.5–4 × 106) were injected into the silkworms, and the silkworms were reared at 30 °C. Survival of the animals was monitored. n = 5/group. (e) Conidia of A. vanbreuseghemii TIMM2789 dermatophytes were cultured at 28 °C for 24 h. Germinated conidia (8 × 106) or samples autoclaved at 121 °C for 15 min (heat-killed germinated conidia) were injected into silkworms, and silkworms were reared at 30 °C. Survival of the animals was monitored. n = 5/group.