Figure 7.

Therapeutic effects of terbinafine on silkworms infected with A. vanbreuseghemii dermatophytes expressing eGFP. Conidia (5 × 10⁶) of A. vanbreuseghemii dermatophytes expressing eGFP were injected into silkworms, followed by injection of terbinafine (20 μg) into the silkworm midgut. The silkworms were reared at 30 °C for 3 days. (a) Small pieces of fat body were placed on glass slides and pressed flat by cover slips. The samples were observed under a microscope (in brightfield, under excitation light, and merged images). (b) The fat body tissue was homogenized by sonication and the centrifuged pellet was suspended in water. The suspended sample (0.5 μl) was dropped on a microscope slide and dried. The samples were observed under a fluorescence microscope. After obtaining an image of the homogenized tissue, the fluorescence intensity of the sample area was determined. Florescence intensity/μg ∙ tissue protein is presented. n = 3/group.