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. 2017 Sep 25;7:12257. doi: 10.1038/s41598-017-12481-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Microbiome diversity of bacterial population in rumen, colon, and cecum samples obtained from control (NA) and treated (AB) steers. (A) The beta-diversity of the bacterial community was measured using Bray-Curtis index. The X and Y axes denotes the samples. The Bray-Curtis index varies between 0 and 1, where 0 means no difference in species between samples and 1 means all the species are different between samples. (B) Principal coordinate analysis (PCA) of the microbial diversity in GI tract of steers showed that the rumen microbiome samples, except for NA-04, clustered together and separated from cecum and colon samples.