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. 2017 Sep 25;7:12257. doi: 10.1038/s41598-017-12481-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Predicted functional profile of the microbiome in the GI tract of the steers. The metagenomic functional analysis was performed in MG-RAST using Subsystems database with maximum e-value at 10⁻⁵ value, minimum percentage identity at 60%, minimum alignment length of 30 amino acids and abundance 100. Treated steers (AB) were given antibiotic feed additives and control steers (NA) were the control animals which received no treatments. The X axis denotes the samples and Y axis denotes the percentage of predominant functional genes present in the sample at level 2 hierarchy.