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. 2017 Sep 25;7:12284. doi: 10.1038/s41598-017-12509-x

Figure 1.

Figure 1

Resveratrol, a red wine polyphenol, increases SHBG production in HepG2 cells. (a) SHBG accumulation in the medium of HepG2 cells treated daily with vehicle, white wine (1 µl/ml) or red wine (1 µl/ml) was measured using an ELISA. (b) Analysis of SHBG mRNA levels in HepG2 cells treated as in A. Human 18 S (h18S) rRNA was amplified as a control. (c) SHBG accumulation in the medium of HepG2 cells treated daily with vehicle or resveratrol 10 µM or 25 µM was measured using an ELISA. (d) Analysis of SHBG mRNA levels in HepG2 cells treated as in C. Human 18 S (h18S) rRNA was amplified as a control. Data points are shown as mean ± SEM of triplicates. *p < 0.05 when compared with the control. (e) HNF-4α protein levels were measured by Western blotting using PPIA as a housekeeping reference protein in control and resveratrol (25 µM) treated HepG2 cells. Data points are mean ± SEM. (f) f PPARγ protein levels were measured by Western blotting using PPIA as a housekeeping reference protein in HepG2 cells treated as in (e). Data points are mean ± SEM.