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. 2017 Sep 25;7:12233. doi: 10.1038/s41598-017-12452-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development pattern of the growth cone. (A) The growth cones are categorized into three subtypes: blunt-ended (collapsed with no visible filopodia or lamellipodia), filopodial (growth cones with numerous filopodia and a small or absent lamellipodial veil), or lamellipodial (well spread growth cones with elaborate lamellipodia). (B) Area of growth cone reached a peak on D9. (C) The percentage of the lamellipodial type growth cone increased before D9 and then decreased (n = 60, ***P < 0.001, *P < 0.05).