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. 2017 Sep 25;7:12261. doi: 10.1038/s41598-017-12434-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro enzymatic activity of sPLA₂-III on phospholipids extracted from the colon. Release of fatty acids (a) and lysophospholipids (b) from colon-extracted phospholipids (10 μM) after incubation for 30 min with the indicated concentrations of recombinant sPLA₂-III (n = 4). Individual lysophospholipid and fatty acid species were evaluated by ESI-MS. Values are mean ± SEM. LPE, lysophosphatidylethanolamine; LPS, lysophosphatidylserine; LPG, lysophosphatidylglycerol. Representative results of two experiments are shown.