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. 2017 Sep 25;7:12288. doi: 10.1038/s41598-017-11620-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of main coverage metrics. Average of all samples analyzed for the four different approaches. (a) Bar plots with 95% confidence interval for the four approaches. Alignment metrics: passing filter (PF) reads; percentage of PF reads that aligned to the reference sequence; and percentage of PF reads that were aligned to the reference sequence with a mapping quality of Q20 or higher signifying. (b) Tukey boxplots: Mean read depth; percentage of bases covered at C1; and percentage of bases covered at C30.