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. 2017 Sep 25;7:12287. doi: 10.1038/s41598-017-12498-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Allele frequencies of the SNPs identified in the targeted sequencing of β-defensin genes in bulls of high and low fertility. The frequency of the alternate allele in bulls of high fertility is shown on the x axis and the alternate allele frequency in bulls of low fertility on the y axis. SNPs which have a difference in SNP frequency of >20% between bulls of high and low fertility are highlighted in red. SNPs with a SNP frequency difference over 20% between groups are more likely to be under selection pressures, than SNPs with low SNP frequency differences.