Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 25;7:12245. doi: 10.1038/s41598-017-12561-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Co-immunoprecipitation and proximity ligation assay of TRPV4 and SK3 proteins. Immunoblotting (IB) for TRPV4 or SK3 in SK3 or TRPV4 immunoprecipitates (IP) of detrusor membrane fraction (n = 4, a). Lane 1, protein standards; lane 2, IB for TRPV4 in detrusor membrane fraction (1 μg); lane 3 IB for TRPV4 in SK3 IP; lane 4, IB for TRPV4 in non-immune rabbit IgG IP of detrusor membrane fraction; lane 5, IB for SK3 in detrusor membrane fraction (1 μg); lane 6, IB for SK3 in TRPV4 IP; lane 7, IB for SK3 in non-immune rabbit IgG IP of detrusor membrane fraction (n = 4, b). Isolated detrusor PDGFRα⁺ cell morphology visualized by DIC microscopy (c). The same cell verified as PDGFRα⁺ by GFP expression. PLA analysis of the same cell using goat anti-TRPV4 and rabbit anti-SK3 antibodies (d).