Fig. 7.

Generation-specific activity of the synthetic GAL1 promoter in gal80Δ background (strain WP274) and the resulting noise dynamics during aging. a Generational fluorescence levels for N = 82 ON mother cells of the GAL1 promoter edited and GAL80 gene deleted strain (WP274). In addition to the deletion of GAL80 gene, this strain is also edited by introducing nucleosome disfavoring sequences (Methods) onto the GAL1 promoter driving YFP. Each generation has four measurements or more. All the mother cells used in the study have replicative lifespan of 10 generations or more. b Mean generational fluorescence levels for the cells described in a. c The aging axis is divided into non-overlapping windows of two generations, and the mean and SD of fluorescence levels across all available cells inside each window are plotted. The error bars denote SD, the number of data points used for the SD quantification are 10 or above. d CV values of individual cells inside each window. e Mean and SEM of the CV’s across the cell population as shown in d. f Fano factor values of individual cells inside each window. g Mean and SEM of the Fano factors across the cell population as shown in f. For the SEM quantifications in e, g, the number of data points used is at least 10