Fig. 2.

WP760 blocks melanoma cells in G2/M phase and induces apoptosis. Cell cycle analysis of nine cell lines treated with 100 nM WP760 (for 24 h) by flow cytometry (a). Representative histograms of cell cycle distribution (treated - WP vs. controls - CONTR) are shown. Cell cycle distribution presented as mean ± SD of at least three independent experiments (b); * P-values <0.05, ** P-values <0.01 (Student’s t-test). Western blotting analysis of caspase 3 cleavage in WP760-treated cell lines (c). Cells were treated with 100 nM (or 50 nM for 1205Lu) for 24 (upper) or 48 h (lower); HSP70 (loading control). Figure shows representative results of at least two independent experiments