Fig. 4.

Effect of WP760 and Actinomycin D on global transcription level in melanoma cell lines. Cells were treated with various concentrations of WP760 for 24 h. RNA transcription level was determined by measuring the signal of fluorescently labeled 5-ethynyl uridine (EU) incorporated into nascent RNA. Asterisks indicate statistically significant differences, P-values < 0.05 (Fisher test) (a). Effect of WP760 on subcellular localization of B23 protein in control and WP760-treated (100 nM; 24 h) WM793B cells (b). Western blots of total p53 and p21 in WP760-treated (100 nM; 24 h) lines (c). WP760 increases the amount of p53 and p21 significantly and consistently in 8 out of 9 human melanoma cell lines tested; HSC70 (loading control). The panel shows representative results of two independent experiments