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. 2017 Sep 21;8:1821. doi: 10.3389/fmicb.2017.01821

FIGURE 5.

FIGURE 5

The lpxXL mutant of ORS278 is drastically impaired in symbiosis with Aeschynomene plants. Comparison of the growth of A. evenia (A) and A. indica (B) (aerial part), non-inoculated (N.I.) or inoculated with ORS278, lpxXL or BRADO4680 mutants. (C,D) Box plots representation of the quantification of acetylene reduction activity (ARA) and number of nodules per plant inoculated with ORS278 (white), lpxXL (gray) or BRADO4680 (dark gray) mutants in A. evenia (C) and A. indica (D). The experiment was carried out in duplicate with 10 plants per condition; Tukey’s honestly significant difference test indicates a significant effect (∗P < 0.1; ∗∗P < 0.01). (E–H,L,M) Whole roots of A. evenia (E–H) and A. indica (L,M) inoculed with ORS278 (E,F,L) or the lpxXL mutant (G,H,M); (F,H) cut nodules; scale bars: 2 mm. (I–K,N–P) Confocal microscopy observations of nodules from A. evenia (I–K) and A. indica (N–P) elicited by ORS278 (I,N), or the lpxXL mutant (J,K,O,P); scale bars: 20 μm. (P) The white arrow indicates a plant defense reaction.