FIGURE 6.

Use of the P_tet inducible system in vivo. 12 mice were treated with clindamycin to disrupt the natural flora. All mice were subsequently infected with the same culture of P_tet-spo0A grown overnight without ATc (infectious dose = 1 × 10⁷ CFU). The mice were then split into two groups of 6, one of which received 1% ethanol in their drinking water, and the other 100 μg/ml ATc. Feces were collected at the time indicated post-infection and enumerated for total and heat-resistant CFUs. Data plotted for each animal individually with mean indicated (^∗p ≤ 0.05; ^∗∗p ≤ 0.01 by a multiple Student’s t-test using the Holm-Sidak method). Dotted line represents the limit of detection.