Table 1.

Within-run precision, extraction efficiency and total error in dog VS.

	Precision			Extraction efficiency						± Total error (%)	Total error acceptance criteria (%)
	Mean concentration (pg/ml)	Within-run precision (%CV)	Precision acceptance criteria (%)	Expected concentration after spiking (pg/ml)	Mean of measured concentration after spiking (pg/ml)	Within-run precision (%CV)	Precision acceptance criteria (%)	% Spiking recovery	± Relative error (%)
VS1	17.6	18.1	≤25	67.6	54.9	3.6	≤20	81	19	22.6	≤30
VS2	22.2	0.8	≤25	55.5	70.1	2.5	≤20	126	26	28.5	≤30
VS3	48.6	2.2	≤25	90.1	72.4	4.9	≤20	80	20	24.9	≤30

Acceptance criteria in this species were as follows: the absolute mean bias % relative error (RE) and the %CV should be ≤ 20% of the nominal value at each concentration level (≤25% at the interval between 1x and 3xLLOQ, i.e., 16.4–49.2 pg/ml). The total error should not exceed 30% (40% at the interval around the LLOQ). Values out of the acceptance range are indicated in bold.