Table 2.

Within-run precision, extraction efficiency and total error in cat VS.

	Precision			Extraction efficiency						± Total error (%)	Total error acceptance criteria (%)
	Mean concentration (pg/ml)	Within-run precision (%CV)	Precision acceptance criteria (%)	Expected concentration after spiking (pg/ml)	Mean of measured concentration after spiking (pg/ml)	Within-run precision (%CV)	Precision acceptance criteria (%)	% Spiking recovery	± Relative error (%)
VS1	21.8	18.5	≤25	41.8	47.8	10.5	≤20	114	14	24.5	≤40
VS2	26.4	11.4	≤25	45.6	34.9	1.2	≤25	77	23	24.2	≤40
VS3	112.2	9.0	≤20	145.2	141.1	5.8	≤20	97	3	8.8	≤30

Acceptance criteria in this species were as follows: the absolute mean bias % relative error (RE) and the %CV should be ≤ 20% of the nominal value at each concentration level (≤25% at the interval between 1x and 3xLLOQ, i.e., 16.7–50.1 pg/ml). The total error should not exceed 30% (40% at the interval around the LLOQ).