Table 8.
Between-run precision in dog VS, established from three independent runs on different days, using two different lots of Enzolifesciences EIA kits.
| Run 1 | Run 2 | Run 3 | Between-run mean concentration (pg/ml) | Between-run precision (%CV) | Precision acceptance criteria (%) | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean concentration (pg/ml) | Within-run precision (%CV) | Precision acceptance criteria (%) | Mean concentration (pg/ml) | Within-run precision (%CV) | Precision acceptance criteria (%) | Mean concentration (pg/ml) | Within-run precision (%CV) | Precision acceptance criteria (%) | ||||
| VS A | 26.1 | 3.7 | ≤25 | 39.7 | 31.9 | ≤25 | 52.7 | 23.0 | ≤20 | 39.5 | 33.7 | ≤25 |
| VS B | 73.5 | 10.5 | ≤20 | 67.3 | 23.8 | ≤20 | 79.9 | 27.7 | ≤20 | 73.5 | 8.6 | ≤20 |
| VS C | 132.8 | 20.0 | ≤20 | 124.7 | 24.0 | ≤20 | 125.9 | 14.3 | ≤20 | 127.8 | 3.4 | ≤20 |
Values out of the acceptance range (i.e., if CV >20%, or >25% at the interval between 1x and 3xLLOQ) are indicated in bold.