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. 2017 Sep 21;8:1645. doi: 10.3389/fpls.2017.01645

Figure 1.

Figure 1

Molecular analysis of TaMYC1 transcripts and deduced protein. (A) The six transcript isoforms (I–VI) identified for TaMYC1. The number of exons (represented by filled boxes) covered by the six isoforms varied from five (Isoform IV) to nine (Isoform V). (B) Amino acid sequence comparison between TaMYC1 (deduced from Isoform III) and two representative bHLH regulators of anthocyanin biosynthesis, RS from maize and Ra from rice. The three domains (bHLH-MYC_N domain, HLH domain and ACT-like domain) conserved among known bHLH TFs regulating anthocyanin biosynthesis are underlined. The GenBank accession numbers of the three sequences are NP_001106073 (RS), AAC49219 (Ra), or KX867111 (TaMYC1). (C) Localization of TaMYC1-GFP fusion protein in the nucleus of Arabidopsis protoplast. As a control, the free GFP protein was distributed throughout the cytoplasm of the protoplast. The images shown were taken under a confocal microscope using green (for GFP fluorescence) or red (for chlorophyll fluorescence) filters or under bright field (BF). The merged images depicted more clearly the relative positions of GFP and chlorophyll fluorescence in the photographed protoplasts.