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. 2017 Sep 21;8:1645. doi: 10.3389/fpls.2017.01645

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Copyright © 2017 Zong, Xi, Li, Chen, Zhang, Liu, Liu, Wang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Induction of anthocyanin biosynthesis by TaMYC1 in combination with ZmC1. The expression constructs of ZmC1, ZmR, and the six transcript isoforms of TaMYC1 (Isoforms I–VI) were delivered into the coleoptile cells of the white-grain wheat Opata with particle bombardment in appropriate combinations or singularly. The presence of red colored cells in the bombarded coleoptiles indicates induction of anthocyanin biosynthesis. Of the six different types of transcripts of TaMYC1, only Isoform III induced anthocyanin biosynthesis in combination with ZmC1. The results shown are typical of three independent assays.