Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 21;10:299. doi: 10.3389/fnmol.2017.00299

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Niebert, van Belle, Vogelgesang, Manzke and Niebert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Subcellular localization of serotonin (5-HT) receptors in vitro and in vivo. (A) When expressed recombinantly in N1E-115 cells, 5-HT_1A/2C/4/6/7 produce a clear membrane staining. In contrast, 5-ht_5a/5b produce a distinct intracellular staining with weak (5-ht_5a) or no (5-ht_5b) membrane staining. Artificially truncating the other 5-HT receptors (here shown exemplarily for 5-HT₇) produces the same intracellular pattern observed for 5-ht_5a/5b. Scale bars represent 10 μm. (B) The intracellular localization of 5-ht_5b is not an artifact of recombinant expression, but can be visualized in vivo. Immunohistological staining of murine brainstem (01) from hemizygous Mecp^+/− mice against 5-ht_5b (see “Materials and Methods” Section) reveals upon magnification (02) the same clustered intracellular localization (03) as seen in cells. Scale bars as indicated. Bregma is ~ −6.5. Abbreviations: Amb, Nucleus ambiguus; IOPr, inferior olive, principal nucleus; Sp5, spinal trigeminal tract. (C) Co-localization of 5-HT receptor 5-ht_5b (purple) with cellular compartment markers (green) in primary hippocampal neurons. Neurons between DIV 9 to DIV 11 were transfected with fluorescently labeled 5-HT receptor 5-ht_5b and compartment markers for membrane (GAP-43), endoplasmic reticulum (ER; recognition sequence of calreticulin), mitochondria (recognition sequence of cytochrome C-oxidase), lysosomes (Lamp-1) and endosomes (rab5). The strongest co-localization is with the endosomal marker. Scale bars in all images are 20 μm.