Figure 6.
Identification of possible interaction domains between 5-HT1A (green) and 5-ht5b (purple). N1E-115 cells were transiently transfected with two hybrid receptor variants labeled with eGFP or mCherry. (A) Co-expression of full length 5-HT1A and full length 5-ht5b lead to strong internalization of 5-HT1A. (B) Replacing the C-terminal domain of 5-ht5b with the C-terminal domain of 5-HT1A (HRV-15) did not change the amount of interaction. (C) Expressing 5-HT1A together with a hybrid 5-HT1A where the 3rd intracellular loop and the last two TMDs are from 5-ht5b (HRV-17), the interaction is greatly reduced or even abolished. (D) Expressing 5-HT1A together with a hybrid receptor composed of the first three TMDs of 5-HT1A and the last four TMDs of 5-ht5b (HRV-18) show interaction as the two full length receptors.