Table 1.

Comparison of RNA yield and quality from rumen solid (RS) with different initial grinding masses and different masses used for RNA isolation.

Grinding mass (g)	Isolation mass (g)	RNA yield (μg RNA/g RS)	LS/SS rRNA ratio	RIN value
3.0	0.6	21.9 ± 16.9	1.95 ± 0.13	7.3 ± 0.2
	0.3	195.5 ± 220.4	1.68 ± 0.21	6.9 ± 0.8
2.0	0.6	17.4 ± 13.5	1.85 ± 0.13	5.0 ± 1.7
	0.3	123.5 ± 153.2	1.55 ± 0.38	6.0 ± 2.0

^∗Data represents mean and standard error for at least 3 replicates per experiment.