Table 2.

Comparison of RNA yield and quality from rumen solid (RS) with different amount of TRIzol solution.

TRIzol volume	RNA yield (μg RNA/g RS)	23S/16S rRNA ratio	RIN value
10×	20.0 ± 4.0	1.85 ± 0.07	7.3 ± 0.0
7.5×	34.1 ± 31.5	1.75 ± 0.35	7.25 ± 0.2
5×	36.5 ± 33.4	1.65 ± 0.21	6.55 ± 0.2

^∗Data represents mean and standard error for at least 3 replicates per experiment.