TABLE 1 .
Deletion loci and complements generated in this study
| Strain type | Description |
|---|---|
| WT | Wild-type deletion background, ΔBT2275 FUdRr, intact thiamine biosynthetic and transport operonsa |
| Deletion mutants | |
| ΔBioThi | Deletion of thiamine biosynthetic operon thiSEGCHF-tenI (BT0647–BT0653 [797661–803616]) |
| ΔOMthi | Deletion of putative outer membrane, TonB-dependent thiamine transporter BT2390 (2977834–2980058) |
| ΔpnuT | Deletion of putative inner membrane thiamine transporter BT2396 (2992218–2992778) |
| ΔΔBioThi-OMthi | Deletion of BT0647–BT0653 and BT2390 |
| ΔΔBioThi-pnuT | Deletion of BT0647–BT0653 and BT2396 |
| ΔΔTransport | Deletion of BT2390 and BT2396 |
| ΔΔΔ | Deletion of BT0647–BT0653, BT2390, and BT2396 |
| Complementation constructs | |
| ΔBioThi+pBioThi | BT0647–BT0653 complemented in trans |
| ΔΔBioThi-OMthi+pBioThi | BT0647–BT0653 complemented in trans |
| ΔΔBioThi-pnuT+pBioThi | BT0647–BT0653 complemented in trans |
| ΔΔTransport+pOMthi | BT2390 complemented in trans |
| ΔΔTransport+ppnuT | BT2396 complemented in trans |
| ΔΔTransport+ppnuT+OMthi | BT2396 and BT2390 complemented in trans |
a
For details, see reference 33.