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. 2017 Sep 21;10:301. doi: 10.3389/fnmol.2017.00301

Figure 3.

Figure 3

Downregulation of SBF2-AS1 inhibited GBM cell-driven angiogenesis in vitro. (A) Expression levels of SBF2-AS1 were detected by qRT-PCR in glioma tissues (Grades I–II, seven cases; Grade III, six cases; Grade IV, six cases) and NBTs (five cases). Data represent mean ± s.d. (n = 3, each), #P and *P < 0.05,**P, &&P, and ##P < 0.01. (B) Expression levels of SBF2-AS1 were detected by qRT-PCR in normal human astrocytes, U87 and U118 cell lines. Data represent mean ± s.d. (n = 4), **P < 0.01. SBF2-AS1 was knockdown in U87 and U118 cells. Effects of GBM cell-conditioned medium on ECs were detected. (C) ECs cell viability was measured by CCK-8 assay. (D) ECs migration was measured by Transwell assay. (E) ECs tube formation was measured by Matrigel tube formation assay. Data represent mean ± s.d. (n = 4, each). *P < 0.05, **P < 0.01. Scale bar represents 30 μm.