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. 2017 Sep 21;10:301. doi: 10.3389/fnmol.2017.00301

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Copyright © 2017 Yu, Zheng, Liu, Xue, Shen, Zhao, Li and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NFAT5 regulated SBF2-AS1 at transcriptional level. (A) Linear regression analysis was performed to each individual NFAT5 and SBF2-AS1 expression, P < 0.01. (B) NFAT5 was knockdown in U87 and U118 cells. SBF2-AS1 levels in U87 and U118 cells were measured by qRT-PCR. Data represent mean ± s.d. (n = 4, each). ^**P < 0.01. (C) Schematic representation of the human SBF2-AS1 promoter region. Chromatin immunoprecipitation PCR products for putative NFAT5-binding sites and an upstream region not expected to associate with NFAT5 are amplified by PCR using their specific primers (n = 3, each).