Figure 7.

miR-338-3p downregulated EGFL7 by targeting its 3′-UTR. (A) The binding site on EGFL7 3′-UTR and the seed region of miR-338-3p. (B) Relative luciferase activity in HEK293T cells that co-transfected pmirGLO-EGFL7-3′-UTR-Wt or Muts and agomir-338-3p were determined. Data represent mean ± s.d. (n = 3, each). ^**P < 0.01. Agomir-338-3p was transiently transfected in U87 and U118 cells that were stably overexpressed EGFL7 with or without 3′-UTR. Effects of co-transfection of agomir-338-3p and EGFL7 with or without 3′-UTR on EGFL7 protein level (C), secretion (D) were measured. Effect of miR-338-3p on EGFL7 mRNA level (E) was measured. Data represent mean ± s.d. (n = 4). ^*P and ^#P < 0.05, ^&&P < 0.01.