Fig. 2.

STAT3 and NF-κB activations are required for IL-6-induced COX-2 expression. THP-1 cells were pre-treated with S3I-201 alone or in combination with kaempferol (Kae) followed by induction with IL-6 (A). The cells were pre-treated with BAY-11 (B) before induction with IL-6. Expressions of p-STAT3 (Tyr 705), STAT3, p-NF-κB (Ser536), NF-κB and COX-2 were studied by western blots. The band intensities were quantitated and data are presented as mean ± SEM of three independent experiments.