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. 2017 Sep 21;8:1647. doi: 10.3389/fpls.2017.01647

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Copyright © 2017 Li, Zhou, Wei, Chen, You and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of transcriptomes in Ws, CBF2-ox and CBF3-ox plants. (A) Venn diagram representing the total number of differentially expressed genes (DEGs) between CBF2-ox or CBF3-ox and Ws. CBF-ox up-regulated (log₂ ≥ 1, FDR ≤ 0.01) or down-regulated (log₂ ≤ -1, FDR ≤ 0.01) genes were shown. (B) Hierarchical cluster analysis of the DEGs. The heat-map was drawn with DEGseq of R package. (C) Verification of CBF regulon expression in Ws, CBF2-ox and CBF3-ox plants. Eight genes were selected for comparison of RNA-seq and qRT-PCR results. For RNA-seq data, ^∗∗ represents FDR ≤ 0.01. The data of qRT-PCR were the means of three technical replicates ± SD (^∗∗P ≤ 0.01; Student’s t-test). Three independent experiments were carried out with similar results.