Table 4.

While increased Th2 transcription factor was found after each DSS treatment cycle, only Il4 was increased after acute DSS treatment.

Th2-associated transcription factor and cytokines
Gata3	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.20 ± 0.03	0.90 ± 0.28*	0.24 ± 0.05	0.96 ± 0.17**
Cycle 2	0.35 ± 0.05	1.66 ± 0.27***	0.24 ± 0.06	1.06 ± 0.24*
Cycle 3	0.21 ± 0.03	1.51 ± 0.3***	0.12 ± 0.02	1.23 ± 0.51**
Il4	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.37 ± 0.03	0.27 ± 0.06	0.47 ± 0.08	0.87 ± 0.13*
Cycle 2	0.90 ± 0.2	1.02 ± 0.30	0.68 ± 0.1	0.49 ± 0.13
Cycle 3	1.29 ± 0.22	0.63 ± 0.08*	0.81 ± 0.14	0.57 ± 0.04
Il5	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.13 ± 0.06	0.04 ± 0.02	0.13 ± 0.07	0.13 ± 0.08
Cycle 2	0.77 ± 0.23	0.57 ± 0.23	0.59 ± 0.11	0.35 ± 0.13
Cycle 3	0.93 ± 0.12	0.58 ± 0.12ρ = 0.082	0.76 ± 0.13	0.31 ± 0.05*
Il13	Proximal colon		Distal colon
	Control mice	DSS-treated mice	Control mice	DSS-treated mice
Cycle 1	0.98 ± 0.29	0.51 ± 0.1	0.79 ± 0.12	1.03 ± 0.13
Cycle 2	0.96 ± 0.21	1.11 ± 0.46	0.66 ± 0.16	0.71 ± 0.16
Cycle 3	1.21 ± 0.19	1.27 ± 0.28	0.82 ± 0.2	0.87 ± 0.14

Colons were collected for examination of the mRNA expression level of Th2 transcription factors and associated cytokines. The mRNA expressions of Gata3, Il4, Il5 and Il13 in the proximal- and distal part of the colon are shown. All results are expressed as + SEM, n = 6. ^* indicates the significant different between control and DSS-treated mice.

^*P < 0.05.

^**P < 0.01.

^***P < 0.001.